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Old 05-19-2017, 05:36 AM   #1
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Location: Uppsala

Join Date: Apr 2009
Posts: 5
Default FastQC Kmer, polyA, polyG, polyC, polyT...

Hi all,

I got the Illumina reads from a NGS company. It is a bird genome. The quality control running by fastQC seems very weird. Too many poly kmers as attached.

Also, I have run the assembly, and the result was N50=150, no assembly at all!

I wonder if the reads of the sequencing is problematic??

Thanks in advanced!
Attached Images
File Type: png kmer_profiles.png (36.8 KB, 14 views)

Last edited by wb1016; 05-19-2017 at 06:12 AM.
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