Hello everyone,
i am a junior research. I have worked on the deep sequencing for human miRNA profiling in human plasma samples. I recently prepared the manuscript relating to miRNA profiling obtained by Solexa sequencing.
During the preparation, i have encounter an issue about the sequencing depth. I already looked up the literature. Though i understood what the depth is, i am still confused with this concept.
The common description of coverage is shown as 30X, for example.
So, my question is like that:
How do you depict the coverage of miRNA sequencing data? Is it according to the equation referring to C=LN/G was used to calculate the coverage, where C stands for coverage, G means the haploid genome length, L is the read length, N is the number of reads?
thanks a lot for help.
sincerely,
i am a junior research. I have worked on the deep sequencing for human miRNA profiling in human plasma samples. I recently prepared the manuscript relating to miRNA profiling obtained by Solexa sequencing.
During the preparation, i have encounter an issue about the sequencing depth. I already looked up the literature. Though i understood what the depth is, i am still confused with this concept.
The common description of coverage is shown as 30X, for example.
So, my question is like that:
How do you depict the coverage of miRNA sequencing data? Is it according to the equation referring to C=LN/G was used to calculate the coverage, where C stands for coverage, G means the haploid genome length, L is the read length, N is the number of reads?
thanks a lot for help.
sincerely,
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