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Old 12-18-2017, 01:47 AM   #23
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Location: Russia

Join Date: Feb 2013
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Originally Posted by epistatic View Post
Ok, now we know how to amplify PCR-products. I think primer digestion process consist of two reactions. First, uracil-glycosylase hydrolyze U from 5'-strand and we have nick. I am still wondering what would be second reaction where strand opposite to nick digested. Do you have any ideas?
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