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Old 12-18-2017, 01:53 AM   #24
korostin
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Location: Russia

Join Date: Feb 2013
Posts: 8
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Quote:
Originally Posted by Buckethead84 View Post
Hi,

Has anyone gotten this protocol to work? I have a protocol that uses Phusion U MM (thanks to epistatic) that appears to work, but I haven't tried it without the end repair reaction yet. Do you think the end repair is necessary?
I think end-repair would be useful, especially PNK treatment primers usually are not phosphorilated.
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