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Old 11-21-2014, 11:05 PM   #16
BioKiwi
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Location: KiwiTeritory

Join Date: Sep 2014
Posts: 19
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Quote:
Originally Posted by bunce View Post
Aerosols build in labs over time. In a post PCR area you can use ART tips, UV and gloves but this minimises contamination it won't remove it. A good PCR once 'opened' will generate aerosols with 10^5-10^9 copies of DNA that can travel through HEPA filters and build in a lab.
If I understand you correctly, you are saying that contamination by PCR is inevitable and nothing can be done about it. I have to disagree with you because PCR has been and is and probably will be corner stone of molecular biology.

If I open my PCR tube in a PCR cabinet with outside filtered air pushing through, any aerosol will be pushed away by air flow. Any possible remaining amplicon can be degraded by UV light or cleaned chemically and cabinet will be free from any amplicon. The question is what evidence you have or you can refer to show that usual lab hygiene is not sufficient to prevent PCR contamination. An amplicon that supposedly sticks to lab wall, how likely can be airborne again and land in a PCR tube. In a very slim probability if it lands how that really is going to affect results because the target amplicons will be in billions.

I strongly, would suggest external expert review of ancient DNA set up and practices if they can not control PCR contamination.
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