Dear all,
I am wondering: how much (methodological) advantage would it have to RNAseq in duplicate (i.e. 2 biological replicates) compared to 1 single seq?
I do not have the resources to have 3 replicates (or more). Samples will be pooled set anyhow (from 6 subjects), but can pool into 1 (=6 subjects) or 2 (=2x3 subjects) samples per condition.
Of course, statistics will be still sub-optimal when have only 2 measures per condition compared to >=3 -- but on the other hand, the reliability should be better. I am mainly interested in differential expression, btw.
Thanks!
I am wondering: how much (methodological) advantage would it have to RNAseq in duplicate (i.e. 2 biological replicates) compared to 1 single seq?
I do not have the resources to have 3 replicates (or more). Samples will be pooled set anyhow (from 6 subjects), but can pool into 1 (=6 subjects) or 2 (=2x3 subjects) samples per condition.
Of course, statistics will be still sub-optimal when have only 2 measures per condition compared to >=3 -- but on the other hand, the reliability should be better. I am mainly interested in differential expression, btw.
Thanks!
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