Hello, I am new to this forum. I am writing this thread because I wanted to know what would be the best way to go about mapping RNA seq reads that I have to my reference gneome (hg 19 genome). I have 8 fastq files that I need to map. I am new to this mapping and need help.
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Basic guide: http://en.wikibooks.org/wiki/Next_Ge..._%28NGS%29/RNA
TopHat/Cufflinks: http://www.nature.com/nprot/journal/....2012.016.html
If you are a biologist wanting to learn (enough) UNIX to use some of the programs above then part I of this guide: http://korflab.ucdavis.edu/Unix_and_...rl_v3.1.1.html
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by seqadmin
The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...-
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05-06-2024, 07:48 AM -
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by seqadmin
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
Channel: Articles
04-22-2024, 07:01 AM -
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