View Single Post
Old 11-09-2011, 04:03 AM   #2
pmiguel
Senior Member
 
Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
Posts: 2,317
Default

Yes or you could just dilute your DNA TruSeq adapters 20x.

We have gone below 1 ug. Possibly to 100 ng, with no modifications to the protocol. But we generally size select prior to enrichment PCR. So adapter dimers are not that much of an issue.

--
Phillip
pmiguel is offline   Reply With Quote