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Old 11-18-2011, 01:32 AM   #1
Anjani
Junior Member
 
Location: Lyon, France

Join Date: Nov 2011
Posts: 1
Default loss of library DNA in the last purification, need help

Hi,
I'm doing library preparation for SOLiD 5500. I have no problem during the process (size selection, dA tail, adaptor ligation etc), until the purification step after amplifying the library. We have no DNA left after purification . I used Agencourt AMPure beads for purification. Is anybody has the same problem and know is/are the cause(s), and know how to solve it? Our group has the same problem before.
Many thanks
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