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Old 09-06-2012, 08:50 PM   #2
jinpingzhang
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Location: Houston

Join Date: Mar 2012
Posts: 10
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Hi, Anjani,
i do not know what kind of your library, i try to contribute some minds.Ok, Agencourt AMPure beads is excellent product for purefication for DNA and RNA, they have RNA Clean beads for clean RNA specificlly. even i already try single product, it is work very good.
i just gess they use PEG8000 and NaCl as bead suspention buffer. maybe the concentration is 13%PEG/1.25M NaCI/10mM MgCI2, we dont care that. if you dont like that, try these way:62% ethanol in final concentration, plus 500mM NH4OAc in final concentration. for example: if your DNA/RNA solution is 100ul, add 100% Ethanol 221ul and 36ul 5M NH4OAc to total 357ul. on magnetic rack 1min, discard original bead supernatant, suspend in your made solution. at room temp mix well and incubate less 5minutes, then set in a magnetic rack, discard supernatant, dry to near crack pellet, dont over dry!! elute your H2O or TE.
remember please, 55%-62% ethanol will give your gold range for purefication process, depending your experiment request.
good luck
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