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Old 10-26-2012, 02:23 PM   #1
aquleaf
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Location: Baltimore

Join Date: Mar 2010
Posts: 38
Default Distinguish real duplicates from those generated by PCR

Hi All.

Sorry to bother you. Is there a way to distinguish duplicates caused by high sequencing depth from the PCR artefacts?

One more question. Is it necessary to remove the duplicates in the transcription factor ChIP-Seq? In our TF ChIP-Seqs, It is often to see a high duplication level. It is not the case in the controls. I guess the duplicate is caused by high sequencing depth not by the PCR artefacts.

Wish your help! Thanks very much!


Best, Mei
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