Hello,
I have recently inherited some RAD data sequenced on an Illumina GAII platform. The protocol used was similar to Baird et al. (2008; see end of post for link). The samples were barcoded and sequenced in lanes containing libraries from two samples (each prepared sample should have had the same DNA concentration).
My question is related to the sequencing output. I have found a lot of variation between the read number for each individual sample; there can be up to a threefold difference in the number of reads between individuals.
Could anyone on this forum explain to me the reasons that there would be such large differences in read count from each sample?
Thank you!
Baird et al: http://www.plosone.org/article/info:...l.pone.0003376
I have recently inherited some RAD data sequenced on an Illumina GAII platform. The protocol used was similar to Baird et al. (2008; see end of post for link). The samples were barcoded and sequenced in lanes containing libraries from two samples (each prepared sample should have had the same DNA concentration).
My question is related to the sequencing output. I have found a lot of variation between the read number for each individual sample; there can be up to a threefold difference in the number of reads between individuals.
Could anyone on this forum explain to me the reasons that there would be such large differences in read count from each sample?
Thank you!
Baird et al: http://www.plosone.org/article/info:...l.pone.0003376
Comment