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Old 10-05-2015, 04:17 PM   #2
kerplunk412
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Location: Bioo Scientific, Austin, TX, USA

Join Date: Jun 2012
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Your plan sounds perfect. You can probably find a Kapa library prep protocol on their website, so you can use the PCR conditions from that. I think they are something like 98 degree melt and 65 degree anneal. Also, you should be checking the concentration of your PCR-free libraries by QPCR, so the primers from that kit should work as well.
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