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Old 11-11-2011, 01:12 PM   #1
Smriti
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Location: California

Join Date: Mar 2011
Posts: 40
Default Effect of %AT content of genome on shearing

Hi There,

Can anyone help me finding an article/study where effect of %AT content has been related to the shearing efficiency. I sheared Lambda and Plasmodium DNA with same settings. Surprisingly, Plasmodium sheared much faster. Also, I got a wavy slope towards lower base pairs in Plasmodium as compared to Lambda DNA. I am attaching the bioanalyzer curves of both DNA here. Can anyone help me in explaining this behavior. I know that Plasmodium DNA has 80%AT content compared to Lambda (50%).
Attached Images
File Type: jpg Plasmodium-250bp-1.jpg (96.7 KB, 10 views)
File Type: jpg Lambda-300bp-1.jpg (86.3 KB, 5 views)
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