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Old 07-26-2011, 05:30 PM   #1
Location: London, UK

Join Date: Jul 2009
Posts: 21
Default BS-Seq from a single cell?

Hello there,

is anybody attempting BS-Seq from a single cell or, anyway, from very limited amount of material?

Given that there is immunoprecipitation step like for Medip and MBD it should be doable?

Applying a similar logic to the one employed for single-cell transcriptomics, one would convert with bisulphite (quite a straight-forward chemical reaction which should not be impacted by low quantity of DNA), then purify (there might be issues there?) and use a WGA kit?

Any comments/ideas very welcome!
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