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Old 09-04-2013, 06:52 AM   #1
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Location: San Francisco, Ca

Join Date: Mar 2013
Posts: 9
Question Library prep with sheared ssDNA

Does anyone know the integrity/makeup of the 5' and 3' ends of Covaris-sheared ssDNA? I am working on a library prep method for single stranded cDNA and am hoping to ligate adapters post shearing.

My assumption is that the cleavage could not leave intact 5' phosphate and 3' OH. Therefore, I am also wondering about methods to repair these ends that would not require generation of a second strand.
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