View Single Post
Old 09-04-2013, 06:16 PM   #2
Registered Vendor

Join Date: Mar 2013
Posts: 210
Default ssDNA ligation

Check out this article, it should help: Single-stranded DNA library preparation for the sequencing of ancient or damaged DNA.

If you don't follow the approach in the paper, you can use T4 Polynucleotide Kinase to add a 5'- Phosphate and remove 3-phosphoryl groups.

- Genohub
Genohub is offline   Reply With Quote