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Old 09-04-2013, 06:16 PM   #2
Genohub
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Default ssDNA ligation

Check out this article, it should help: Single-stranded DNA library preparation for the sequencing of ancient or damaged DNA.

If you don't follow the approach in the paper, you can use T4 Polynucleotide Kinase to add a 5'- Phosphate and remove 3-phosphoryl groups.


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