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Old 02-25-2014, 03:28 PM   #6
seqsense
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Location: Asia

Join Date: Feb 2014
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Hey, don't forget that unless the sequencing is done on a single cell, which does happen for researching tumour genesis, there will be many, many copies of the DNA already without the need for PCR given the multiple cells that will be extracted in sample preparation.

Besides, there's been comment about the nature of the T issue being a systematic problem that bioinformaticians can't handle well, but PCR always introduces systematic errors by its very nature and also drowns out any hope of identifying any other bases except A, C, G and T.

But now there are five different C's! What else don't we know.
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