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Old 02-25-2014, 03:35 PM   #7
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Originally Posted by seqsense View Post

Besides, there's been comment about the nature of the T issue being a systematic problem that bioinformaticians can't handle well, but PCR always introduces systematic errors by its very nature and also drowns out any hope of identifying any other bases except A, C, G and T.

But now there are five different C's!
I'm sorry but I don't follow any of this.

What is "the T issue"?
What evidence is there to support that there are more than five nitrogenous bases and how does PCR suppress this evidence?
What are the "five different C's"
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