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Old 02-18-2016, 06:37 AM   #2
Location: Atlanta, GA

Join Date: Jan 2015
Posts: 25

I have not used the NEBnext kit, but I do regularly use the Kapa BioSciences kit for IonTorrent libraries. It is significantly less expensive than the Life Tech kit and I get much much better results with it. Not including cleanup or barcodes - just the cost for the fragmentation & library kit - I calculate Kapa comes to $29 per sample while Life Tech is $80. Additionally, Kapa provides a much easier workflow.

If you contact Kapa, they will often give you a discount on a "sample" kit to try. See product KK8300 & KK8301. KK8602 is the fragmentation kit, sold separately. I have not purchased their barcodes, as we're swimming in BIOO barcode kits.
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