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Old 08-07-2017, 07:44 AM   #1
candida utilis
Junior Member
 
Location: Taiwan

Join Date: May 2017
Posts: 5
Default DNase treatment for SMART Seq2

I notice that DNase I treatment is not included in the SMART Seq2 protocol. May I know how efficient Oligo-dT30VN will bind to mRNA only? Is there any possibility that Oligo-dT30VN can also amplify DNA from genomic DNA, if there is no DNase I treatment?

Thanks
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