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Old 08-07-2017, 09:33 AM   #2
cmbetts
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Location: Bay Area

Join Date: Jun 2012
Posts: 114
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There's a couple reasons not to worry about it, but the primary ones are:
1) The denaturation temperature before RT isn't high enough to melt high MW gDNA
2) Even if gDNA was primed, the template switching mechanism requires the RT to hit a 5' end and neither the RT nor the PCR polymerase are anywhere processive enough to worry about capturing whole chromosomes.
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