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Old 05-11-2018, 05:14 AM   #2
Location: Brazil

Join Date: Aug 2015
Posts: 92

they should be... Once you've passed the 2-minute minimum incubation time, then the tubes are supposed to be stable for 3 hours (Qubit dsDNA HS Assay guide, page 2), although personally it never happened that I had them them sit for more than half an hour prior to reading them, so I can't speak from personal experience.
What kind of issues are you experiencing? Do you notice readings drop, or raise during time? Or do the readings just fluctuate? How do the calibrators perform at the end of the readings?
Or is your issue that at the end of the experiment, the proportion of libraries is uneven? In this case, besides quantity, are they even in quality?
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