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Old 08-14-2019, 06:31 AM   #5
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Location: Eugene, OR

Join Date: May 2013
Posts: 521

Have you thought about using PacBio for long amplicons instead of tagmenting and trying to re-assemble? Each amplicon could get multiple polymerase passes for a high-quality consensus, and be sequenced multiple times for an even better merged consensus. It would definitely be cheaper than Nextera XT preps and a MiSeq run.
Providing nextRAD genotyping and PacBio sequencing services.
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