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Old 08-18-2019, 09:42 PM   #7
Carcharodon
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Location: Honolulu, HI

Join Date: Jul 2015
Posts: 40
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Quote:
Originally Posted by microgirl123 View Post
Well. . . it's actually an old Stratagene qPCR instrument that will allow you to do a single endpoint read so it's only used as a "plate" reader now.

I'm not sure how well Qubit reactions would work in a plate since there's not a good way to really vortex the samples, but I haven't experimented. I half the volume of the reaction in the strip tubes both to save reagent costs and to allow more room for mixing.
I use the Biotium AccuClear system, and for that you simply mix the DNA with the dye/buffer mixture by pipetting and allow it to sit for ~ 5 mins. I would bet good money that the same approach would work with the Qubit reagents.
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