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  • cuffdiff: same run but different FPKM, your thoughts?

    Hello,
    I was wondering how cuffdiff would differ if I run it on the same data.
    Here are the fpkm of 4 runs for a gene: 38.2168 2.50089 32.3986 39.0589
    Same runs about another gene: 46.8446 37.5176 61.7281 33.5432

    Do you experience the same phenomenon?

    How can we trust these FPKM if it is variating so much?

    Thx for your thoughts.
    Thibault

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