Tweet
Pippin Prep for RNA?
For those of you familiar with the Pippin Prep, currently used for automated DNA size selection and band capture, your thoughts on whether or not Sage Science should commercialize a series of cassettes for RNA too? What RNA applications are of greatest interest? Thanks for your thoughts and help!
-
-
If these were denaturing gels that would work on strand denatured DNA as well, I think they would be very useful.
Subtext of a recent thread
is that short ssDNA/adapters may be annealing to longer library products -- preventing their removal via size-selection on dsDNA products. If the size selection were done on ssDNA there is no where for these short contaminants to "hide" and they should be removed.
--
Phillip -
Phillip - We can manufacture any standard precast gel chemistry, including denaturing gels that should enable this. Thanks for the link and the idea. I will discuss it with our team.
GaryComment
-
we would be interested in RNA cassettesComment
-
-
Seems like you have a ready-made list of beta testers right here in this very thread!
Comment
-
I agree with pmiguel. We see effects similar to the ones in the cited thread, and I think a denaturing gel to size select ssDNA fragments would be very helpful.Comment
-
RNA on Pippin Prep
Sounds good everyone. I will discuss this application with the team and Sage. Thanks for your thoughts, interest and willingness to test RNA cassettes. - GaryComment
-
Yes, send them to Purdue and we will test them on our Pippin prep.
I think there are lots of otherwise perfectly good libraries that are getting hosed by small "hitchhiker" amplicons that can't be removed with double-stranded size-selection methods. Maddening!
Well, there is always normal denaturing PAGE. But it always seems like such a hassle to extract the sample from polyacrylamide gels.
--
PhillipComment
-
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM
Comment