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Old 01-09-2018, 01:27 AM   #26
korostin
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Location: Russia

Join Date: Feb 2013
Posts: 8
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Quote:
Originally Posted by HiroMishima View Post
Hi all,

In the following paper, we described a technique to prepare Illumina libraries using AmpliSeq amplicons:
J Hum Genet https://www.nature.com/articles/s10038-017-0392-9
PubMed https://www.ncbi.nlm.nih.gov/pubmed/29279608
Thanks for links to your article!

1. As I understand from your article you decided to avoid cuttting ampliseq primers. Why?
2. Why you used uracil depletion technique instead of U-passing with Phusion U from Thermo with average dNTP's? In total U-containing part of library after PCR would be tiny (and would not form clusters on flowcell)
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