SEQanswers - View Single Post - Targeted quantitative RNA-sequencing with interlibrary concordance and reduced cost

KristenC · 11-26-2013, 01:21 PM

Hi Tom,

I really enjoyed reading your paper! You touch on a couple of very important issues present in targeted RNA-sequencing.
I am currently working on a targeted RNA-sequencing assay, and am exploring possibilities for using reference genes to normalize the counts. Since your assay does not even need reference genes, I was wondering if you at some point looked into it and maybe dismissed it for certain reasons? Could you comment on why you moved away from possible adaptations of the more traditional RNA-seq approaches?

Kind regards,

Kristen

11-26-2013, 01:21 PM	#2
KristenC Junior Member Location: New Zealand Join Date: Nov 2013 Posts: 7	Interesting! Hi Tom, I really enjoyed reading your paper! You touch on a couple of very important issues present in targeted RNA-sequencing. I am currently working on a targeted RNA-sequencing assay, and am exploring possibilities for using reference genes to normalize the counts. Since your assay does not even need reference genes, I was wondering if you at some point looked into it and maybe dismissed it for certain reasons? Could you comment on why you moved away from possible adaptations of the more traditional RNA-seq approaches? Kind regards, Kristen