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Old 08-10-2009, 09:28 AM   #1
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Location: Durham, UK

Join Date: Aug 2009
Posts: 1
Default Number of runs required SNP detection transcriptome

I am just planning my first use of Solexa/Illumina sequencing.
I'm hoping to use this to sequence the transcriptome of a non-model small mammal in order to identify SNPs. RNA from several different individuals and tissues would be pooled for sequencing.
Can anyone tell me, approximately how many sequencing runs I would need to obtain adequate coverage in order to do this?
Thanks very much,
Tombre is offline   Reply With Quote