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Old 12-08-2015, 06:37 AM   #1
Senior Member
Location: Oklahoma

Join Date: Sep 2009
Posts: 411
Angry 3000/4000 read 2

Anybody here with a 3000/4000 seen poor quality read 2's lately? I have attached example plots from 4 previous runs below. It seems to be library agnostic.

I've been lead to understand that there is an escalating investigation internal to Illumina into possible chemistry issues. They're not comp-ing runs until lot numbers are given for the failed runs, I know that.

Just wondering how far the problem extends.
Attached Images
File Type: jpg 151203_Bioo_PCR-free.jpg (68.7 KB, 52 views)
File Type: jpg 151105_Kapa_hyper.jpg (49.2 KB, 34 views)
File Type: jpg 150930_Kapa_hyper.jpg (49.9 KB, 25 views)
File Type: jpg 151022_Nextera.jpg (44.2 KB, 34 views)
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