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Old 12-10-2015, 06:18 AM   #7
GW_OK
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Location: Oklahoma

Join Date: Sep 2009
Posts: 411
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Latest run has finished. Two large Bioo NextFlex pools across respective halves of the flowcell.

Read 2 still looks weak. One thing of note is that the top surface looks better than the bottom. That being said neither surface's read 2 look nearly as good as read 1.

luc, thanks for posting. I'm glad I'm not the only one seeing this.

I've also included a Q30 plot from Illumina's "HiSeq 4000: TruSeq PCR-Free (NA12878)" publicly shared run on BaseSpace as an example of what I imagine good runs are supposed to look like.
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