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Old 12-10-2015, 10:48 AM   #8
Senior Member
Location: US

Join Date: Dec 2010
Posts: 466

Hi Brian,

We have seen the problems on the HiSeq 4000 the last 6 weeks, and since quite a while on our Miseqs (we are underclustering considerably for these to get any usable data).

Originally Posted by Brian Bushnell View Post
Luc, which platform (and chemistry, and software) are you having trouble with?

FYI, our HiSeq 2000/2500 machines seem fine, which is one of the reasons I have recommended against "upgrading" to HS3000 or 4000 (from which I have never seen good data). As far as I know, our NextSeq is fine too, but our MiSeqs are outputting junk for 2x300bp amplicon runs.

Last edited by luc; 12-10-2015 at 05:13 PM.
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