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Old 01-08-2016, 03:50 PM   #12
luc
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Location: US

Join Date: Dec 2010
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Ours tend to look unfortunately like your last example - we have not seen significant differences between customer prepped libraries our own libraries and also PCR-free libraries.
At what concentration are you loading the PCR-free libraries and and how many clusters did you sequence?

Thanks!
Quote:
Originally Posted by GW_OK View Post
Two more since then have given mixed results. The first was a Bioo NextFlex PCR-free and is perhaps one of the best runs we've had on this machine. The Q30 plot is attached (the one with black boxes).

After that run another, with a mix of library types, gave poorer performance. The Q30 plot for this is also attached, this time with blue boxes.
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