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Old 06-23-2016, 09:30 PM   #4
Pavo
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Location: Israel

Join Date: Jun 2016
Posts: 1
Default On the same matter

Hi,
We consider using nextseq instead of hiseq for sequencing small RNAs in C. elegans, but the rumor has it that the results tend to have serious problems with:
A. strong biases for the first nucleotides (or even more specifically - G as the 1st nuc)
B. hard time sequencing repetitive reads.
Have you experienced any of these problems?
Thanks in advance,
Pavo
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