View Single Post
Old 04-19-2018, 02:42 AM   #2
nucacidhunter
Senior Member
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,171
Default

Methylation sensitive enzymes are more useful for plants as their genome contains large repetitive regions due to duplication events. PstI is methylation sensitive in plants as in plants methylation normally is in non-CpG context.

Choosing methylation insensitive RE can result in sequencing non-informative tags from repeat regions increasing the cost and has no other consequence. But this will depend on the genome and I do not think it would be a concern for non-plant species.

If you have access to Pippin instrument ddRAD would be a better choice.
nucacidhunter is offline   Reply With Quote