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Old 09-21-2014, 06:05 PM   #1
Nepa10
Junior Member
 
Location: US

Join Date: Jun 2014
Posts: 8
Default tblastn and blastx question

It says that tblastn is more likely to find distantly related sequences than the blast, blasts, and blastp. I wonder why is it? I generally use Blastx.

By definition, tblastn compares query protein sequence with the database after translating each nucleotide sequence into protein using all six reading frames

whereas

Blastx first translates query sequence into amino acids in six reading frames (three forward and three back) then compares the protein sequences with protein databases.

Any insight would be greatly appreciated!

Thanks.
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