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Old 04-19-2016, 04:46 PM   #6
mhgseq
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Location: TX

Join Date: Oct 2015
Posts: 7
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Thank you so much for the info. I am also do the similar short amplicon ( ~100bp + primers+ adaptor). But for the loading, we load much higher concentration for OT2 (200 pM vs the recommend 26 pM), I get 8% empty well and 21% polyclonal.
I am thinking about reducing the loading concentration as you mentioned above. I will certainly try your heat cooled approach.
Do you do the heat cooled before purification or after?
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