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Old 10-06-2010, 03:59 AM   #1
Location: Canada

Join Date: Jun 2010
Posts: 49
Question When is Open reading frame=gene?

Dear All,

We have carried out RNA-seq using Illumina. My question is If I have a transcript of size say 600 amino acids ( =1800bp), then when I get the possible ORFs for that transcript, how can I decide that which ORF is the best candidate to be a putative gene?

Also, to let you know further info, this transcript did not give any similarity to existing proteins from the nearby species. Henceforth, I would like to knw which ORF could be taken as putative candidate novel gene for my sepcies? Sounds tricky to me..

Thanks in advance!!
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