Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Do proteins interfere with NGS or lib prep??

    Hi all,

    I found this forum a week a ago and it has turned out to be useful. The only thing I'm still wondering about is said in the title. I will be doing DNA sequencing on 454 after seq capturing. I suspect that the gDNA that I have after extraction has RNA as a contamination (liver tissue so I expect RNA) and I want to treat the gDNA with RNAse A. But after that I'm not sure how important it is to get rid of the RNAse in the gDNA and by serching around I haven't found a good answer. I'm using salt-extraction and dissolving my gDNA in to H2O. So after treating with RNAse, should I re-extract or just precipitate to get rid of the RNAse-proteins or just let it be?

    Thanks,
    Heidi

  • #2
    Hi Heidi, I would recommend a cleanup of gDNA (columns, beads or phenol ChCl3) before you do library prep. If I got it right, you want to use the DNA for sequence capture before actual sequencing. Having clean DNA in first place would already help a lot in the downstream.

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Strategies for Sequencing Challenging Samples
      by seqadmin


      Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
      03-22-2024, 06:39 AM
    • seqadmin
      Techniques and Challenges in Conservation Genomics
      by seqadmin



      The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

      Avian Conservation
      Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
      03-08-2024, 10:41 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, 03-27-2024, 06:37 PM
    0 responses
    12 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-27-2024, 06:07 PM
    0 responses
    11 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-22-2024, 10:03 AM
    0 responses
    53 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-21-2024, 07:32 AM
    0 responses
    69 views
    0 likes
    Last Post seqadmin  
    Working...
    X