SEQanswers - View Single Post - Hello from Jim Tripp at JGI, Walnut Creek CA

Peppe · 11-26-2012, 04:42 PM

Hello Jim,
I see you are at the JGI and I though to ask you an advice/question. I am performing RNAseq analysis using a yeast whose genome sequence is available on the JGI website. I downloaded both the unmasked genome and the annotation file. I need the annotation file for the cufflink and cuffcompare steps, but the problem that I have is that the downloaded file is in gff and galaxy (the public server for analyzing NGS data) accepts only gtf files. Do you have any contact there that can help me?
Thanks

11-26-2012, 04:42 PM	#2
Peppe Member Location: USA Join Date: Nov 2012 Posts: 11	Hello Jim, I see you are at the JGI and I though to ask you an advice/question. I am performing RNAseq analysis using a yeast whose genome sequence is available on the JGI website. I downloaded both the unmasked genome and the annotation file. I need the annotation file for the cufflink and cuffcompare steps, but the problem that I have is that the downloaded file is in gff and galaxy (the public server for analyzing NGS data) accepts only gtf files. Do you have any contact there that can help me? Thanks