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Old 08-12-2017, 08:08 AM   #1
patkrat
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Location: London

Join Date: Apr 2017
Posts: 13
Default Role/Importance of adapter contamination in cDNA libraries

I have created cDNA libraries from Drosophila melanogaster mRNA using the KAPA mRNA HyperPrep kit. The size distribution on the Bioanalyzer looks good, as I was expecting a mean of ~320 bp. I attached a screenshot of an example trace for one of my samples: My question concerns the two peaks very close to the upper marker (left-hand side): Are these adapter dimers (probably not, given their small size), or left-over primers? If not, what could they be? And, would this pose a problem for sequencing, given how much of them is present compared to the cDNA fragments? Thanks for any help!
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Last edited by patkrat; 08-12-2017 at 09:03 AM.
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