View Single Post
Old 08-12-2017, 08:08 AM   #1
Location: London

Join Date: Apr 2017
Posts: 14
Default Role/Importance of adapter contamination in cDNA libraries

I have created cDNA libraries from Drosophila melanogaster mRNA using the KAPA mRNA HyperPrep kit. The size distribution on the Bioanalyzer looks good, as I was expecting a mean of ~320 bp. I attached a screenshot of an example trace for one of my samples: My question concerns the two peaks very close to the upper marker (left-hand side): Are these adapter dimers (probably not, given their small size), or left-over primers? If not, what could they be? And, would this pose a problem for sequencing, given how much of them is present compared to the cDNA fragments? Thanks for any help!
Attached Images
File Type: png Screen Shot 2017-08-12 at 15.54.48.png (78.0 KB, 42 views)

Last edited by patkrat; 08-12-2017 at 09:03 AM.
patkrat is offline   Reply With Quote