Hello everyone,
we often have the the problem, that we have libraries with small size (ie small RNA), but we don't have enough samples to sequence a complete flowcell. So we were wondering, if it is possible to sequence the libraries with small size on a 100bp HiSeq run and use afterwards just the first 30-40bp. Are there any problems with that (sequencing or cBot) or would a small library size on one lane influence for example other lanes? Has anyone experience with that or any recommendations? I would be happy for all advice.
Thanks Robby
we often have the the problem, that we have libraries with small size (ie small RNA), but we don't have enough samples to sequence a complete flowcell. So we were wondering, if it is possible to sequence the libraries with small size on a 100bp HiSeq run and use afterwards just the first 30-40bp. Are there any problems with that (sequencing or cBot) or would a small library size on one lane influence for example other lanes? Has anyone experience with that or any recommendations? I would be happy for all advice.
Thanks Robby
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