Hello everyone,
This might be a very simple question to all but I am new to this field and want to learn about the high throughput analysis.
I have two cel files generated from Affy HT430MG arrays. One is a control and other is the treated . I want a list of differentially expressed genes in the treatment as compared to the control.
For this , I used the affy package in R/Bioconductor and used rma for normalization and background correction. I got a .txt file with the different expression levels.
But now I want to annotate the probes and find out in what way do they affect certain pathways.
What will be a good approach to go ahead with it.?
Any help is much appreciated.
Thanks a lot,
Himanshu
This might be a very simple question to all but I am new to this field and want to learn about the high throughput analysis.
I have two cel files generated from Affy HT430MG arrays. One is a control and other is the treated . I want a list of differentially expressed genes in the treatment as compared to the control.
For this , I used the affy package in R/Bioconductor and used rma for normalization and background correction. I got a .txt file with the different expression levels.
But now I want to annotate the probes and find out in what way do they affect certain pathways.
What will be a good approach to go ahead with it.?
Any help is much appreciated.
Thanks a lot,
Himanshu
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