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seqgirl123 · 08-02-2019, 10:00 AM

When one is preparing cells for the 10x genomics single cell RNA Seq platform, the manual says to dilute the cells (in media or 1x PBS) into water for a final volume of 46.6ul to achieve your targeted cell recovery. Do users mix their cell suspension in with water, then pipette into the mastermix at this step?

08-02-2019, 10:00 AM	#1
seqgirl123 Member Location: U.S Join Date: Oct 2008 Posts: 76	10x genomics scRNASeq When one is preparing cells for the 10x genomics single cell RNA Seq platform, the manual says to dilute the cells (in media or 1x PBS) into water for a final volume of 46.6ul to achieve your targeted cell recovery. Do users mix their cell suspension in with water, then pipette into the mastermix at this step?