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Old 02-23-2014, 12:10 PM   #1
ryan.england@esr.cri.nz
Junior Member
 
Location: NZ

Join Date: Feb 2014
Posts: 1
Default Ampliseq panel on MiSeq

Hi I have made an Ampliseq panel for use on the PGM, it has 280 targets of sizes between 250-150bp.
We would like to also sequence these same targets on the MiSeq (we are testing to see how the two platforms compare on our types of targets and may possibly buy the one we like best).
Has anyone before used the Ampliseq primer panel to prepare a library for the MiSeq?
Or have any suggestions about which library prep method would be best?

We have looked at the truseq custom amplicon designer but we would prefer to use the ampliseq primer panel we already have. This is due to the cost of purchasing the full truseq amplicon kits (they only coming in 96sample minimums when we really only want to try on around 10 samples to begin with).
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