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Old 02-23-2014, 06:56 PM   #2
bunce
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Location: Perth

Join Date: Sep 2012
Posts: 55
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Hi Ryan,
A very real option for you to -'readapt' the A/P1 Ampliseq products (via PCR) with the illumina p5/p7. You would typically do 12-20 cycles of PCR with the fusion Primers. This would be a cheap and easy way for doing the reaction. It is not, technically, a valid comparison as the MiSeq library would have gone through subsequent PCR (=more error) but.....If you are sequencing microsats you will find the MiSeq data much (MUCH) cleaner. The story is much the same for SNPs. The homopolymer error in the PGM is difficult to cope with when scoring alleles. Happy to discuss this with you 'offline' - drop me a line (michael.bunce 'at' curtin.edu.au). Cheers.
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