View Single Post
Old 03-13-2014, 04:36 PM   #2
luc
Senior Member
 
Location: US

Join Date: Dec 2010
Posts: 431
Default

Apparently simply Taq polymerase can be used for A-tailing at very low concentrations:

Neiman et al 2012:
http://www.plosone.org/article/info%...l.pone.0048616

The published protocol carries out the ligation at 16 degrees overnight, though.
luc is offline   Reply With Quote