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Old 06-08-2014, 11:06 PM   #1
vl80
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Location: Asia

Join Date: Nov 2012
Posts: 34
Default Nextera XT vs TruSeq PCR free

I have previously used the TruSeq DNA kit (only once) and now need to move on to use either Nextera XT or TruSeq PCR free kits and perform the whole library prep alone for the first time.
I would greatly welcome opinions on which of the two kits have given better results for small genomes (bacterial)

2)
For Nextera XT, is it better not to use the bread normalization and do the traditional quantifications? There seem to be mixed experiences for using bead normalizations.

3) is there anyone using Qubit alone for library quantification? Or is it better to use KAPA qPCR instead or both?

4) This is probably a very naive question,
for Nextera XT with bacterial genomes, is it necessary to sonicate/ nebulize to get a larger size fragment and then use the kit based tagmentation than the kit alone.

Thanks a lot
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