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Old 09-23-2015, 01:55 AM   #7
cement_head
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Location: Oxford, Ohio

Join Date: Mar 2012
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Basically one would design PCR primers such that the 3' portion is complementary to the target, and the 5' half contains the barcode and the Illumina adaptor sequences for the instrument you are using. You can get the Illumina sequences from the sequence letter. Just use Google to find online resources to help design the primers.

Try this first: https://www.google.com/url?sa=t&rct=...NSQ0L-KGPUg4QQ
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